Effect of HDAC1 silencing on apoptosis of squamous cell carcinoma of skin via regulating STAT3 signaling pathway / 中国病理生理杂志

AIM:To investigate the effect of histone deacetylase 1(HDAC1)silencing on apoptosis of squa-mous cell carcinoma of skin.METHODS:Skin squamous cell carcinoma A431 cells were transfected with HDAC1 small interfering RNA(HDAC1 siRNA)or small interfering RNA negative control(siRNA NC).The expression levels of HDAC1 in transfected cells were detected by RT-PCR and Western blot.The cell viability was measured by MTT assay, and the apoptosis was analyzed by flow cytometry.The protein levels of STAT3,p-STAT3 and cleaved caspase-3 were de-termined by Western blot.The inhibitor of STAT3 signaling pathway was used to treat the A 431 cells transfected with HDAC1 siRNA.The cell viability was detected by MTT assay,the apoptosis was analyzed by flow cytometry,and the pro-tein levels of STAT3,p-STAT3 and cleaved caspase-3 were determined by Western blot.RESULTS: HDAC1 siRNA in-hibited the expression of HDAC1 at mRNA and protein levels in the A431 cells.After interfering with the expression of HDAC1,the cell viability and the protein level of p-STAT3 in the cells decreased,while the apoptotic rate and the protein level of cleaved caspase-3 in the cells were increased.After treatment with the inhibitor of STAT3 pathway,the viability of A431 cells transfected with siRNA and the protein level of p-STAT3 decreased,while the apoptotic rate and the protein le-vel of cleaved caspase-3 in the cells were increased.CONCLUSION: Interference with HDAC1 expression may regulate the STAT3 signaling pathway to inhibit the viability of skin squamous cell carcinoma cells,thus promoting the apoptosis of squamous cell carcinoma of skin.

A novel mutation in DKC1 gene in a patient with dyskeratosis congenita / 中华皮肤科杂志

Objective To detect the mutation in DKC1 gene in a patient with dyskeratosis congeni- ta.Methods Fifteen exons of DKC1 gene were amplified by polymerase chain reaction (PCR),and the products were screened for mutations by denaturing high performance liquid chromatography (DHPLC) technology,then DNA sequencing was performed for abnormal exons as shown by DHPLC.The gene muta- tions were verified within 100 unrelated male individuals without dyskeratosis congenita.Results An ab- normal DHPLC elution peak was found in exon 12 of DKC1 gene of the patient,but not in other family members or normal individuals.DNA sequencing showed a 1236G→T transition in DKC1 gene in the pa- tient,which resulted in a 412W→C substitution in DKC1.No mutation was found in other family members and normal individuals.Conclusion The 1236G→T transition in the patient is a novel mutation in DKC1 gene,which could be a causative factor of dyskeratosis congenita.